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feeder cell layer hs  (ATCC)


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    Structured Review

    ATCC feeder cell layer hs
    Primary bone marrow cells co-cultured <t>with</t> <t>HS-5</t> marrow stromal cells. (A) Visualization of the culture in 1 μM Hoechst 33258 (blue) and 0.5 μM NucView (green, indicating activated caspase 3 in cells undergoing apoptosis. (B) Image analysis inverting the blue channel from part A to differentiate HS-5 cells (large diffuse; red arrow) from bone marrow cells (small, roundish, and intense).
    Feeder Cell Layer Hs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/feeder cell layer hs/product/ATCC
    Average 97 stars, based on 307 article reviews
    feeder cell layer hs - by Bioz Stars, 2026-05
    97/100 stars

    Images

    1) Product Images from "Efficacy evaluation of glasedgib Sonic Hedgehog pathway inhibition with or without inotuzumab in B-ALL cells using a new co-culturing system model and a validated chemosensitivity assay"

    Article Title: Efficacy evaluation of glasedgib Sonic Hedgehog pathway inhibition with or without inotuzumab in B-ALL cells using a new co-culturing system model and a validated chemosensitivity assay

    Journal: bioRxiv

    doi: 10.64898/2026.05.07.723573

    Primary bone marrow cells co-cultured with HS-5 marrow stromal cells. (A) Visualization of the culture in 1 μM Hoechst 33258 (blue) and 0.5 μM NucView (green, indicating activated caspase 3 in cells undergoing apoptosis. (B) Image analysis inverting the blue channel from part A to differentiate HS-5 cells (large diffuse; red arrow) from bone marrow cells (small, roundish, and intense).
    Figure Legend Snippet: Primary bone marrow cells co-cultured with HS-5 marrow stromal cells. (A) Visualization of the culture in 1 μM Hoechst 33258 (blue) and 0.5 μM NucView (green, indicating activated caspase 3 in cells undergoing apoptosis. (B) Image analysis inverting the blue channel from part A to differentiate HS-5 cells (large diffuse; red arrow) from bone marrow cells (small, roundish, and intense).

    Techniques Used: Cell Culture

    (A) Experimental design: primary B-ALL blasts were recovered from cryostorage and briefly allowed to recover in co-culture with HS-5 stromal support. Viable blasts were plated for chemosensitivity testing in co-culture and exposed to treatments for four days. Hematopoietic cells were mechanically isolated and assessed for viability using CellTiter-Glo. (B) Relative viability: dose-dependent responses to glasdegib are in seen within the hematopoietic compartment of co-cultured cells (Patients 1 and 2) but not in HS-5 stromal cells alone. Unique patients have differential responses to inotuzumab alone, but co-treatment sensitizes blasts to the effect of glasdegib.
    Figure Legend Snippet: (A) Experimental design: primary B-ALL blasts were recovered from cryostorage and briefly allowed to recover in co-culture with HS-5 stromal support. Viable blasts were plated for chemosensitivity testing in co-culture and exposed to treatments for four days. Hematopoietic cells were mechanically isolated and assessed for viability using CellTiter-Glo. (B) Relative viability: dose-dependent responses to glasdegib are in seen within the hematopoietic compartment of co-cultured cells (Patients 1 and 2) but not in HS-5 stromal cells alone. Unique patients have differential responses to inotuzumab alone, but co-treatment sensitizes blasts to the effect of glasdegib.

    Techniques Used: Co-Culture Assay, Isolation, Cell Culture

    The SHH gene expressions by four primary B-ALL patient samples in different culture conditions. Each panel represents a different patient sample, and each color corresponds to the SHH gene indicated in the legend. The x-axes represent the treatment conditions: negative control (Co DMSO), 20 μM glasdegib (Co Glas 20), and 20 μM glasdegib + 10 ng/mL inotuzumab (Co Glas 20 INO). The y-axes represent the expression of GLI1, GLI3, SMO , and PTCH1 after four days of HS-5 stromal cell co-culture. All expression values were represented by method with GAPDH as the internal control and treatment with DMSO as the fold-change calibrator.
    Figure Legend Snippet: The SHH gene expressions by four primary B-ALL patient samples in different culture conditions. Each panel represents a different patient sample, and each color corresponds to the SHH gene indicated in the legend. The x-axes represent the treatment conditions: negative control (Co DMSO), 20 μM glasdegib (Co Glas 20), and 20 μM glasdegib + 10 ng/mL inotuzumab (Co Glas 20 INO). The y-axes represent the expression of GLI1, GLI3, SMO , and PTCH1 after four days of HS-5 stromal cell co-culture. All expression values were represented by method with GAPDH as the internal control and treatment with DMSO as the fold-change calibrator.

    Techniques Used: Negative Control, Expressing, Co-Culture Assay, Control



    Similar Products

    feeder cell layer hs  (ATCC)
    97
    ATCC feeder cell layer hs
    Primary bone marrow cells co-cultured <t>with</t> <t>HS-5</t> marrow stromal cells. (A) Visualization of the culture in 1 μM Hoechst 33258 (blue) and 0.5 μM NucView (green, indicating activated caspase 3 in cells undergoing apoptosis. (B) Image analysis inverting the blue channel from part A to differentiate HS-5 cells (large diffuse; red arrow) from bone marrow cells (small, roundish, and intense).
    Feeder Cell Layer Hs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/feeder cell layer hs/product/ATCC
    Average 97 stars, based on 1 article reviews
    feeder cell layer hs - by Bioz Stars, 2026-05
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Primary bone marrow cells co-cultured with HS-5 marrow stromal cells. (A) Visualization of the culture in 1 μM Hoechst 33258 (blue) and 0.5 μM NucView (green, indicating activated caspase 3 in cells undergoing apoptosis. (B) Image analysis inverting the blue channel from part A to differentiate HS-5 cells (large diffuse; red arrow) from bone marrow cells (small, roundish, and intense).

    Journal: bioRxiv

    Article Title: Efficacy evaluation of glasedgib Sonic Hedgehog pathway inhibition with or without inotuzumab in B-ALL cells using a new co-culturing system model and a validated chemosensitivity assay

    doi: 10.64898/2026.05.07.723573

    Figure Lengend Snippet: Primary bone marrow cells co-cultured with HS-5 marrow stromal cells. (A) Visualization of the culture in 1 μM Hoechst 33258 (blue) and 0.5 μM NucView (green, indicating activated caspase 3 in cells undergoing apoptosis. (B) Image analysis inverting the blue channel from part A to differentiate HS-5 cells (large diffuse; red arrow) from bone marrow cells (small, roundish, and intense).

    Article Snippet: Patients’ cells were cultured in vitro with support of a feeder cell layer HS-5 stromal cells (ATCC CRL-11882).

    Techniques: Cell Culture

    (A) Experimental design: primary B-ALL blasts were recovered from cryostorage and briefly allowed to recover in co-culture with HS-5 stromal support. Viable blasts were plated for chemosensitivity testing in co-culture and exposed to treatments for four days. Hematopoietic cells were mechanically isolated and assessed for viability using CellTiter-Glo. (B) Relative viability: dose-dependent responses to glasdegib are in seen within the hematopoietic compartment of co-cultured cells (Patients 1 and 2) but not in HS-5 stromal cells alone. Unique patients have differential responses to inotuzumab alone, but co-treatment sensitizes blasts to the effect of glasdegib.

    Journal: bioRxiv

    Article Title: Efficacy evaluation of glasedgib Sonic Hedgehog pathway inhibition with or without inotuzumab in B-ALL cells using a new co-culturing system model and a validated chemosensitivity assay

    doi: 10.64898/2026.05.07.723573

    Figure Lengend Snippet: (A) Experimental design: primary B-ALL blasts were recovered from cryostorage and briefly allowed to recover in co-culture with HS-5 stromal support. Viable blasts were plated for chemosensitivity testing in co-culture and exposed to treatments for four days. Hematopoietic cells were mechanically isolated and assessed for viability using CellTiter-Glo. (B) Relative viability: dose-dependent responses to glasdegib are in seen within the hematopoietic compartment of co-cultured cells (Patients 1 and 2) but not in HS-5 stromal cells alone. Unique patients have differential responses to inotuzumab alone, but co-treatment sensitizes blasts to the effect of glasdegib.

    Article Snippet: Patients’ cells were cultured in vitro with support of a feeder cell layer HS-5 stromal cells (ATCC CRL-11882).

    Techniques: Co-Culture Assay, Isolation, Cell Culture

    The SHH gene expressions by four primary B-ALL patient samples in different culture conditions. Each panel represents a different patient sample, and each color corresponds to the SHH gene indicated in the legend. The x-axes represent the treatment conditions: negative control (Co DMSO), 20 μM glasdegib (Co Glas 20), and 20 μM glasdegib + 10 ng/mL inotuzumab (Co Glas 20 INO). The y-axes represent the expression of GLI1, GLI3, SMO , and PTCH1 after four days of HS-5 stromal cell co-culture. All expression values were represented by method with GAPDH as the internal control and treatment with DMSO as the fold-change calibrator.

    Journal: bioRxiv

    Article Title: Efficacy evaluation of glasedgib Sonic Hedgehog pathway inhibition with or without inotuzumab in B-ALL cells using a new co-culturing system model and a validated chemosensitivity assay

    doi: 10.64898/2026.05.07.723573

    Figure Lengend Snippet: The SHH gene expressions by four primary B-ALL patient samples in different culture conditions. Each panel represents a different patient sample, and each color corresponds to the SHH gene indicated in the legend. The x-axes represent the treatment conditions: negative control (Co DMSO), 20 μM glasdegib (Co Glas 20), and 20 μM glasdegib + 10 ng/mL inotuzumab (Co Glas 20 INO). The y-axes represent the expression of GLI1, GLI3, SMO , and PTCH1 after four days of HS-5 stromal cell co-culture. All expression values were represented by method with GAPDH as the internal control and treatment with DMSO as the fold-change calibrator.

    Article Snippet: Patients’ cells were cultured in vitro with support of a feeder cell layer HS-5 stromal cells (ATCC CRL-11882).

    Techniques: Negative Control, Expressing, Co-Culture Assay, Control